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It is conceivable that the existence of calcium ion has effect to strength of membrane. That is, it is conceivable that the existence of calcium has effect to chemical evolution for birth of life.

　 　
　(a) after 31minutes　　　　　　　　　　　　　　(b) after 1hour 59minutes

　 　
　(c) after 2hours 32minutes　　　　　　　　　 　(d) after 2hours 48minutes

　 　　　
　(e) after 3hours 19minutes　　　　　　　　　　 (f) after 28hours 30minutes
　　Fig.21 Formation of bubbles in the carbonated water (50cc) that was mixed with iron powder (5g) and calcium carbonate (CaCO3: 5g)


4.1.c　Effects of calcium sulfite (CaSO4) to the iron carbide bubble
　The sulfuric acid is included to sea water. So, calcium sulfite of CaSO4 is formed by the chemical bond between calcium and sulfuric acid. It is considered that the first life was born in the sea. We checked the effect of calcium sulfite for the first life as follows. The calcium sulfite (CaSO4: 5g) was mixed with the carbonated water (50cc) that includes with iron powder (5g). The result of this experiment is shown in Fig.23.



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